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Abstract The importance of seeds as virus vehicles for long-distance dissemination makes essential the availability of adequate methods of analysis to guarantee the quality of seed lots. To improve...
Serum samples should generally be diluted at least 1:50 in order to minimize backgrounds caused by non-specific antibody binding. 3. To dilute the sample 1:100, add 1 part sample to 99 parts General Serum Diluent. For example, add 10 μL …
Note: The ELISA seed extract can also be used to perform the SE-qPCR in the ISHI-Veg test: Detection of Infectious ToBRFV in Tomato and Pepper Seed. SAMPLE AND SUB-SAMPLE SIZE The recommended minimum sample size is 3,000 s …
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Liver sample (20 mg) was put into Eppendorf tube (1.5 mL) and internal standard solution (20 μL) was added. Two small steel balls were added into the tube, and the sample was grind in rapid grinding machine for 2 min.
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An automatic sample rapid grinding machine (Jingxin Industrial Development Co., Ltd., Shanghai, China) was used for tissue homogenization. A 0.1-g sample of hepatopancreas tissue from each group was placed in 0.9 ml ice-cold 0.86% sodium chloride solution, and then centrifuged at 3500 g at 4 °C for 15 min. Supernatants were transferred to …
Research in the IDM is led by over 34 independent principal investigators in the basic, clinical and public health sciences, and has a strong translational focus. Grant and contract funding is sourced from the US National Institutes of Health, the Bill & Melinda Gates Foundation, The Wellcome Trust, EDCTP, the South African Medical Research Council, the National Research …
The ATM Stone Grinder is a water cooled, high speed machine for quickly plane grinding samples in a specimen holder. The coarse grit grinding stone turns at 1700 rpm to plane grind a typical batch of samples in less than 30 seconds.
A DAS-ELISA assay may be used to pre-screen a seed extract. The test is complete if no virus is ... sample spiking, or experimental comparisons. ... 2.2. Process seed extracts within 4 hours after grinding. 3. Controls 3.1. Choose one of the PPCs and NPCs listed. 3.2. Depending on the amount of infectious virus in the PPC, adjust the quantity ...
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Grinding machine to a prepareed sample 2) Weigh one gram of the prepared sample into a plastic centrifugal tube. Add 19 mL of the Sample extracting medium into the tube. Mix them well to prepare a uniform dispersion (Note 1). 3) Set the centrifugal tube horizontally in a shaking machine and shake it
What to prepare 4.1 Methane 70% 4.2 grinding machine 4.3 scales 4.4 Blender or shaker 4.5 glass filter And dilute the extract. 4.6 Filter paper No. 4 4.7 micro pipettes of 50-200 and 100-1000 μl with pipette tip 4.8 MicroELISA Reader machine reads at a wavelength of 450 nm
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The tissues were lysed with Trizol (Invitrogen, Thermo Fisher, Shanghai, China) by automatic sample rapid grinding machine following previous methods .Total RNA was reverse transcribed using the Transcriptor first strand cDNA synthesis kit (Roche, Basel, Switzerland) and analyzed on Roche Light Cycler 480 with light cycler 480 SYBR Green I master.
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